Operation principle
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Optical. Traditional microscopes, which work based on the use of lenses and other optical elements. They allow you to provide high image quality and a good magnification factor, while they do not depend on electricity (except that batteries may be needed for the backlight system). This type of microscope uses traditional eyepieces, but there are some models that allow you to connect an external camera and display the image on a computer display. Also note that this is the only principle used in stereoscopic models (see "Type")
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Digital. Microscopes of this type are actually digital cameras, supplemented with powerful magnifying optics. The image from such a camera must be displayed on the screen; some models have their own displays, others do not have displays and need to be connected to a computer/laptop. The advantage of the first variety is independence from external equipment, the advantages of the second option are compactness and relatively low cost. At the same time, it should be noted that in terms of magnification, most digital microscopes are inferior to optical ones, and this principle is not suitable for a stereoscopic image.
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Optical-digital. Microscopes that combine the features of optical and digital models (see relevant paragraphs). Such models differ from "purely digital" devices by more advanced optics, with a revolving head and a
...high magnification; from optical ones — with a built-in camera and using a screen as an eyepiece (traditional eyepieces are not used in optical-digital models).Magnification
The range of magnifications provided by the device is from minimum to maximum.
The magnification of the microscope is calculated by the formula "the magnification of the eyepiece is multiplied by the magnification of the objective." For example, a 20x objective with a
10x eyepiece will give a magnification of 10*20 = 200x. Modern microscopes can be equipped with multi-objective turrets, zoom lenses (see below) and interchangeable eyepieces — so that in most models the magnification can be adjusted. This allows you to adjust the device to different situations: when you need to consider small details, a high degree of magnification is used, but to expand the field of view, the magnification must be reduced.
Detailed recommendations on optimal multiplicities for different tasks can be found in special sources. Here we note that many manufacturers go to the trick and indicate the maximum value of the magnification by the degree of magnification achieved with an additional Barlow lens. Such a lens can indeed give a serious increase in magnification, but it is not a fact that the image will turn out to be of high quality; for more details, see "Complete set".
Eyepiece
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Monocular. An eyepiece with a single lens that can only be viewed with one eye. For obvious reasons, it is only used in biological microscopes (see "Type"). The advantages of monoculars are primarily smaller size and cost than other varieties; in addition, they do not require adjustment for interpupillary distance. On the other hand, constantly looking into the eyepiece with one eye is tiring, so this option is poorly suited for situations where you have to look into the microscope often and for a long time.
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Binocular. Dual eyepiece that can be viewed with both eyes at once. Note that such optics are used not only in stereomicroscopes, originally intended for viewing an object through two lenses (see "Type"), but also in biological microscopes with one lens. The fact is that looking into an optical device with two eyes is much more convenient than with one, while the eyes are less loaded and fatigue does not occur so quickly. Therefore, for serious tasks associated with frequent use of a microscope, binoculars (or trinoculars, see below) are the best option. Such optics cost more than monocular, but this is offset by ease of use.
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Trinocular. A kind of binocular (see the relevant paragraph), supplemented by a third optical channel for a special camera-video eyepiece. Such a camera is usually connected to a PC or laptop; by installing it in the soc
...ket for the third eyepiece, you can take photos and videos, as well as display the image in real time on the computer screen. At the same time, you can look through the microscope in the usual way. Devices with trinoculars are very functional and versatile, but they are complex and expensive.
— LCD screen. The microscope has an LCD screen that replaces the traditional eyepiece. You do not need to bend over to such a device each time to view the image, which is very convenient if observations need to be combined with record keeping and other similar activities. Microscopes of this design usually have a photo and video function, as well as various built-in tools — for example, a scale grid for estimating the size of visible objects, displayed directly on the screen. In addition, the image on the screen can be seen not only by the direct user, but also by everyone who is nearby; such features are indispensable during training sessions, consultations, presentations, etc. On the other hand, such microscopes turn out to be bulky and expensive.
— magnification factor. The magnification provided by the eyepiece. This parameter, along with the lens magnification, affects the overall magnification of the device (see above). The classic option for eyepieces in microscopes is 10x, but higher values \u200b\u200bare also found. The package may include several eyepieces, of different magnification — to change the overall degree of magnification. There is a multiplicity designation with a letter index, for example, WF10x. This means that the eyepiece has an extended field of view (WF — wide, EWF — extra wide, UWF — extra wide).
— Eyepiece tilt. The tilt of the eyepiece determines the position of the observer's head when looking through the microscope and the overall usability. According to this indicator, three main options can be distinguished: fixed angle, adjustable angle, without tilt. The fixed angle is most often 30° or 45° relative to the horizontal, these values are considered the most convenient. In angle-adjustable microscopes, the entire stand, with tube and stage, is fixed to the base with a swivel mount. This is the most convenient option, allowing you to adjust the tilt to your preference, but the mount tends to become loose over time, so it is rarely used in professional microscopes. The third variety — vertical microscopes, without tilt — have not received much distribution: this design is used in some stereoscopic models (see "Type") in order to ensure that the stage remains strictly horizontal (this is important for some work with microscopic objects).
— Rim diameter. The nominal diameter of the eyepiece used in the microscope, as well as the diameter of the hole in the tube, designed to install the eyepiece. Several standard diameters are used in modern microscopes, in particular 23 and 27 mm. In fact, this parameter is necessary, first of all, if you plan to purchase spare or replacement eyepieces for the microscope, or if you already have an eyepiece on the farm, and you need to evaluate its compatibility with this model.
— Diopter adjustment. The range of diopter correction provided in the eyepiece. This correction is used so that a nearsighted or farsighted person can look through the microscope without glasses or contact lenses. In most models with this function, the correction range is about 5 diopters in both directions; this allows the microscope to be used for low to moderate myopia/farsightedness.Object table
The design of the object stage provided in the microscope.
— Stationary. Subject table, fixed motionless; focus in such microscopes is carried out by moving up and down the tube with the objective and the eyepiece. Such systems are simple and inexpensive, but focus while looking through a constantly moving eyepiece is not very convenient. In addition, for advanced biological microscopes (see "Type") with binoculars and trinoculars (see "Eyepiece"), this option is also poorly suited for some design reasons. But the vast majority of stereomicroscopes are equipped with stationary tables — this is the most reasonable design, taking into account the specifics of the application.
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Movable. In microscopes of this type, the entire optical system is fixedly fixed on a tripod, and the stage can be moved up and down to focus the optics. This design is found exclusively in biological microscopes (see "Type"). It is somewhat more complicated and expensive than with a fixed table, but at the same time it is much more convenient: when focus, the eyepiece does not move, which allows you to comfortably adjust the image without looking up. In addition, it is the movable stage that is most suitable for advanced devices with binoculars and trinoculars (see "Eyepiece"), almost all such microscopes have such equipment.
Condenser
Features of the design of the condenser installed in the microscope.
The condenser is part of the illumination system in biological microscopes (see "Type"). This is an optical system that processes the light flux entering the preparation glass in a special way. Different situations may require different ways of doing this; accordingly, different types of condensers can be used in microscopes. However, the most popular nowadays is the simplest Abbe condenser. It ensures the concentration of the beam of light and its uniform distribution over the field of view. Initially, such a device was intended for studies using the bright field method, but it can also be used for phase-contrast observations. The Abbe condenser can be equipped with an iris aperture diaphragm — with its help you can reduce the brightness of the illumination — as well as colour filters.
Other, more specific types of condensers (for example, phase or dark field) are usually purchased separately and are rarely included in the standard microscope equipment.
The characteristics of the condenser may indicate NA — the size of the aperture (active hole) in millimetres, for example, NA \u003d 1.2. This is a rather specific setting; suffice it to say that it is selected by the manufacturer for complete lenses and does not fundamentally affect the choice of a microscope.
Features
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Adjustment of interpupillary distance. The ability to change the distance between the eyepieces in a binocular or trinocular microscope (see "Eyepiece"). For normal visibility, it is necessary that the distance between the lenses of the eyepieces correspond to the distance between the pupils of the user. This distance varies from person to person, so this setting may be required for comfortable use.
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Brightness adjustment. The ability to change the brightness of the backlight — to adjust the lighting to the specifics of the situation. For example, to study a thin transparent preparation in a bright field, high brightness will be unnecessary, but when transilluminating a dense dark object, it is indispensable.
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Illumination according to Keller. The presence of illumination in the microscope according to the Keller system. Such lighting is used exclusively in biological models (see "Type"), it is a sign of a professional level device. The Keller system complicates and increases the cost of the design, in addition, it may require specific settings, but with the right settings, the quality of the lighting is very high, and the image is as reliable as possible. Note that in microscopes there is a so-called. "simplified Keller system", when the settings are set at the factory and cannot be changed; however, in this case, it is precisely the full-fledged,
...adjustable Keller lighting that is meant.
— Photo / video recording. Possibility of photo and video filming of the image visible in a microscope. Features of the implementation of this function in different microscopes may be different. For example, some models need to be connected to a computer, while others can record materials directly to a memory card or other media. Also, the cameras themselves, carrying out the shooting, can be both built-in and removable (see "Packaging" / relevant paragraphs).Connection interfaces
Ways to transfer data to other devices provided in the design of the microscope.
This parameter is relevant primarily for digital and opto-digital models, as well as for individual optical devices equipped with cameras. All described microscopes can be equipped with
AV and
HDMI outputs, universal
USB ports, removable media
card readers, and
Wi-Fi wireless modules. Here is a detailed description of each interface:
— AV output. Analogue output for video signal transmission. It is used primarily for live transmission of images from a microscope camera, and in some models — also for viewing footage stored in memory. Such outputs do not support HD resolutions and, in general, are inferior to HDMI in terms of overall “picture” quality (with the same camera characteristics). On the other hand, specifically for microscopes, these moments are not so often critical; analogue connectors are still quite popular in both conventional video equipment and special equipment; and the implementation of this interface is inexpensive. Therefore, AV outputs can be found even in fairly advanced models.
— HDMI. Digital output for video signal transmission. Similarly, AV can be used both for real-time broadcasting and for using the microscope as a video player when viewing saved materials (if such
...a possibility is provided for in this model at all). At the same time, such outputs are more advanced than analogue AV: HD-quality images (including Full HD and higher) can be transmitted via HDMI, and the signal is very resistant to interference. We also recall that this interface is extremely common in modern video technology — in particular, the presence of at least one HDMI input is almost mandatory for TVs and monitors that support HD standards. On the other hand, the implementation of HDMI is noticeably more expensive, and it makes sense to use it with fairly advanced cameras, which in themselves significantly affect the price of microscopes. Therefore, such outputs can be found mainly in rather expensive and advanced devices.
— USB. Universal connector that allows different applications; a specific set of these options is directly related to the functionality of the microscope. Typical examples of using USB include the following: copying captured photos and videos to a computer or laptop; live image broadcast; remote control via PC / laptop (for example, moving the parent drug); charging the built-in battery, etc. The specific type of USB connector in the microscope may vary, however, usually, an appropriate cable is supplied in the kit for connecting to a standard full-size port.
— Card reader. The device for working with memory cards is usually SD, and in miniature pocket models — microSD. Such cards usually contain materials captured by the camera. In general, this function makes it much easier to copy information to other devices that also have card readers — primarily laptops and PCs; and miniature microSD cards are also supported by smartphones, tablets and other portable gadgets. Anyway, removing the card from the microscope and installing it in another device is often easier and faster than fiddling with a wired or Wi-Fi connection.
— Wi-Fi. A wireless module, which in this case is mainly used to communicate with an external device — such as a smartphone, laptop or PC. A Wi-Fi connection allows you to at least broadcast the image from the camera and copy the photos taken by it, and often also control other functions and settings (light brightness, movement of the driver, etc.). At the same time, the absence of wires provides additional freedom of movement and overall convenience. However, note that the specific communication format may be different, it should be specified separately. So, some models support only direct connection over a relatively short distance (in fact, up to a couple of tens of metres, or even less). Others are able to connect to an external device via the Internet, and here the distance does not play a role — there would be access to the World Wide Web. Still others allow both formats of work. Also note that individual devices with this function do not have their own screens at all and are designed for use with external gadgets; This design makes the microscope as compact and easy to carry as possible.Power source
Methods of nutrition provided in the microscope. Even optical models may require a power source to run the backlight (see above), while for other varieties, power is almost a must. Some models may support multiple power types.
— 230 V network. Connection to a regular 230 V socket. Quite a convenient and practical option, only poorly suitable for portable models (see above).
— USB port. Power supply from the USB connector is often found in digital microscopes (see "How it works"): the device is powered from the same connector through which it is connected to a computer or other external screen. And in optical models, such power supply can be provided in addition to the 230 V network described above. Note that USB ports, among other things, are also found in laptops and other portable devices, which makes it possible to use such microscopes even if there are no outlets nearby. This is especially useful for portable devices (see above).
— Accumulator. Powered by its own built-in battery, in some cases — non-removable. This option makes the microscope completely autonomous and allows you to use it even in the complete absence of external power sources nearby. On the other hand, this moment is relevant mainly for portable models, and then only in some cases, and the built-in battery noticeably affects the weight, dimensions and price of the device. Therefore, purely cordless microscopes are extremely rare, more often this method of power supply is pro...vided in addition to the 230 V network or USB (see above) — as a spare in case of problems with external power.
— Batteries. Another type of autonomous power supply, along with the batteries described above. The presence of a battery compartment is cheaper than the built-in battery, but the batteries themselves have to be purchased separately — and either regularly buy disposable cells, or pay a rather large amount for batteries and a charger for them. In addition, the quality of batteries is highly dependent on the specific brand, and not all cells can normally “start” the microscope and provide an acceptable battery life. Therefore, such power, like battery power, is rare in its pure form, more often it complements the connection to a 230 V network or USB.
In box
Additional equipment supplied with the microscope.
— Camera. In this case, we mean a
removable camera installed either on the main optical channel (to use an external screen as an eyepiece), or on the third additional channel of the trinocular (see "Eyepiece"). In addition, there are also built-in cameras (see the relevant paragraph). Some models supplied without a camera allow you to purchase it separately, but this configuration option is generally more convenient.
— Adapter for smartphone. A device that allows you to install a smartphone on a microscope so that the camera of the device “sees” the image in the eyepiece. Thus, you can take photos and videos on your smartphone, as well as use its screen as an eyepiece — for example, if you want to show the image to several people at once.
— A set of accessories and preparations. A set of accessories for working with a microscope. Such a set usually includes at least slides and coverslips; in addition to them, the kit can be supplied with preparation tools, various auxiliary compounds (resin for gluing, oils and liquids for immersion lenses), as well as ready-made preparations for testing the capabilities of the microscope and initial training in working with it.
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Lens Barlow. An additional lens that is installed in front of the eyepiece and changes the overall magnification — usually upwards, but vice versa is
...also possible. To calculate the overall magnification when using such optics, you need to multiply the initial magnification of the device by the magnification of the lens: for example, a 200x microscope with a 1.6x Barlow lens will give 200 * 1.6 = 320x magnification. This is partly why Barlow lenses have a very low magnification — even it gives a significant increase in magnification. The second reason is that it makes sense to increase the overall magnification only up to a certain limit — above this limit, the optics will only stretch the image without increasing the detail. Actually, in many microscopes, this is exactly what happens if you set the device to the maximum magnification and install a Barlow lens. So this device should be considered more as a tool for adjusting the magnification at medium magnification, and not as a way to increase the maximum magnification.
— Cover / case. Case for storage and transportation of the microscope. Covers are called soft cases, they are mainly designed to protect against pollution; cases are made of hard materials, they are more bulky, but they are also able to protect the device from shocks and shocks.